Distinction of leukemia patients' and healthy persons' serum using FTIR spectroscopy

Daping Sheng; Xingcun Liu; Weizu Li; Yuchan Wang; Xianliang Chen; Xin Wang

doi:10.1016/j.saa.2012.09.072

Distinction of leukemia patients' and healthy persons' serum using FTIR spectroscopy

Spectrochim Acta A Mol Biomol Spectrosc. 2013 Jan 15:101:228-32. doi: 10.1016/j.saa.2012.09.072. Epub 2012 Sep 29.

Authors

Daping Sheng¹, Xingcun Liu, Weizu Li, Yuchan Wang, Xianliang Chen, Xin Wang

Affiliation

¹ National Synchrotron Radiation Laboratory, University of Science and Technology of China, and The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, China.

PMID: 23099176
DOI: 10.1016/j.saa.2012.09.072

Abstract

In this paper, FTIR spectroscopy was applied to compare the serum from leukemia patients with the serum from healthy persons. IR spectra of leukemia patients' serum were similar with IR spectra of healthy persons' serum, and they were all made up of proteins, lipids and nucleic acids, etc. In order to identify leukemia patients' serum and healthy persons' serum, the H1075/H1542, H1045/H1467, H2959/H2931 ratios were measured. The H2959/H2931 ratio had the highest significant difference among these ratios and might be a useful factor for identifying leukemia patients' serum and healthy persons' serum. Furthermore, from curve fitting, the RNA/DNA (A1115/A1028) ratios were observed to be lower in leukemia patients' serum than those in healthy persons' serum. The results indicated FTIR spectroscopic study of serum might be a useful tool in the field of leukemia research and diagnosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Female
Humans
Leukemia / blood*
Leukemia / diagnosis
Male
Middle Aged
Serum / chemistry*
Spectroscopy, Fourier Transform Infrared / methods*