β2-Adrenergic receptor solutions for structural biology analyzed with microscale NMR diffusion measurements

Angew Chem Int Ed Engl. 2013 Jan 2;52(1):331-5. doi: 10.1002/anie.201205474. Epub 2012 Oct 24.

Abstract

Microcoil NMR measurements were performed to determine the final composition of solutions of the β(2)-adrenergic receptor (β(2)AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β(2)AR. Standards are established for the reproducible preparation of G-protein-coupled receptor solutions for crystallization trials and solution NMR studies.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Diffusion
  • Humans
  • Micelles
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • Receptors, Adrenergic, beta-2 / chemistry*
  • Solutions

Substances

  • Micelles
  • Receptors, Adrenergic, beta-2
  • Solutions