Identification of dairy lactic acid bacteria by tRNAAla-23S rDNA-RFLP

J Microbiol Methods. 2012 Dec;91(3):380-90. doi: 10.1016/j.mimet.2012.10.003. Epub 2012 Oct 16.

Abstract

The aim of this study was to evaluate the potential of target tRNA(Ala)-23S ribosomal DNA for identification of lactic acid bacteria strains associated with dairy ecosystem. For this purpose tRNA(Ala)-23S ribosomal DNA Restriction Fragment Length Polymorphism (tRNA(Ala)-23S rDNA-RFLP) was compared with two widely used DNA fingerprinting methods - P1 Random Amplified Polymorphic DNA (RAPD), (GTG)5 repetitive extragenic palindromic PCR (rep-PCR) - for their ability to identify different species on a set of 10 type and 34 reference strains. Moreover, 75 unknown isolates collected during different stages of Grana Padano cheese production and ripening were identified using tRNA(Ala)-23S rDNA-RFLP and compared to the RFLP profiles of the strains in the reference database. This study demonstrated that the target tRNA(Ala)-23S rDNA has high potential in bacterial identification and tRNA(Ala)-23S rDNA-RFLP is a promising method for reliable species-level identification of lactic acid bacteria (LAB) in dairy products.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification
  • Bacteria / genetics*
  • Bacteria / isolation & purification*
  • Bacteria / metabolism
  • Bacterial Typing Techniques / methods*
  • Cheese / microbiology*
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics*
  • Lactic Acid / metabolism*
  • Phylogeny
  • Polymorphism, Restriction Fragment Length*
  • RNA, Ribosomal, 23S / genetics*
  • RNA, Transfer, Ala / genetics*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 23S
  • RNA, Transfer, Ala
  • Lactic Acid