Abstract
We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended-focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amyloid plaques in the brain parenchyma and vasculature and requires no staining of the alzheimeric sample under investigation. xfOCM also opens the possibility to perform minimally invasive studies of β-amyloid pathology in vivo, without the use of labeling methods, which potentially confound experimental findings.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alzheimer Disease / genetics
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Alzheimer Disease / metabolism
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Alzheimer Disease / pathology
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Amyloid beta-Peptides / chemistry*
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Amyloid beta-Peptides / genetics
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Animals
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Cerebral Amyloid Angiopathy / genetics
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Cerebral Amyloid Angiopathy / metabolism
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Cerebral Amyloid Angiopathy / pathology*
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Disease Models, Animal*
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Fourier Analysis
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Humans
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Mice
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Mice, Transgenic
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Microscopy, Confocal / instrumentation
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Microscopy, Confocal / methods
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Neuroimaging / instrumentation
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Neuroimaging / methods
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Plaque, Amyloid / genetics
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Plaque, Amyloid / pathology
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Staining and Labeling
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Tomography, Optical Coherence / instrumentation
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Tomography, Optical Coherence / methods*