Purpose: The aim of this in vitro study was to verify the interference of previous application of sodium hypochlorite in the bond strength to dentin irradiated with Er:YAG laser (80 mJ, 2 Hz, 25 J/cm(2)), using two adhesive systems with different solvents (Adper Single Bond2 - 3M ESPE [SB] and Prime&Bond 2.1 - Dentsply [PB].
Study design: Ninety standardized dentin disks were treatment with phosphoric acid etching (PA), PA and 10% sodium hypochlorite (SH), Er:YAG laser (ER), ER and 10% SH. After surface preparation, samples received SB or PB followed by composite resin Z250 (3M ESPE)(n = 10). Tensile bond strength test was performed (0.5 mm/min) 24 h after bonding procedure and one treated dentin disk of each group was selected to scanning and transmission electron microscopy (SEM and TEM) observations. Bond strength test data were evaluated by three-way ANOVA and Tukey (P < 0.05) tests.
Results: Tested systems had similar bonding behavior when applied with conventional method. SH produced no improvement in bond strength. Phosphoric acid etching either associated with SH, or not, provided higher bond strength values than ER. SEM showed typical ablation pattern on dentin after ER irradiation. SH application after PA promoted greater porosity in intertubular dentin exposing secondary tubules. SH used after ER irradiation showed more evident peritubular dentin. TEM results showed that both PA and ER exposed inter- and peri-tubular dentin collagen fibers.
Conclusions: Er:YAG irradiation had a negative influence on SB adhesive, and in both methods, association of SH did not completely remove collagen fiber network, but altered its configuration.
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