Cloning and functional verification of the Candida milleri HIS3 gene encoding imidazoleglycerol phosphate dehydratase

J Microbiol Biotechnol. 2012 Oct;22(10):1441-5. doi: 10.4014/jmb.1207.07064.

Abstract

The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.5% amino acid identity to Torulaspora delbrueckii IGPD, followed by that to Saccharomyces cerevisiae (79.6%). The cloned HIS3 gene complemented a his3 mutation in S. cerevisiae, suggesting that it encodes a functional IGPD in C. milleri CBS 8195. A new auxotrophic marker is now available for acid-tolerant yeast C. milleri.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Candida / enzymology*
  • Candida / genetics*
  • Candida / growth & development
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Enzyme Activation
  • Enzyme Assays
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal*
  • Genetic Complementation Test
  • Hydro-Lyases / genetics*
  • Hydro-Lyases / metabolism
  • Molecular Sequence Data
  • Open Reading Frames
  • Phenotype
  • Saccharomyces cerevisiae / genetics
  • Sequence Analysis, DNA
  • Sequence Analysis, Protein
  • Sequence Homology, Amino Acid

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Hydro-Lyases
  • imidazoleglycerolphosphate dehydratase