Abstract
The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.5% amino acid identity to Torulaspora delbrueckii IGPD, followed by that to Saccharomyces cerevisiae (79.6%). The cloned HIS3 gene complemented a his3 mutation in S. cerevisiae, suggesting that it encodes a functional IGPD in C. milleri CBS 8195. A new auxotrophic marker is now available for acid-tolerant yeast C. milleri.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Candida / enzymology*
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Candida / genetics*
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Candida / growth & development
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Cloning, Molecular
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DNA, Fungal / genetics
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Enzyme Activation
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Enzyme Assays
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Genes, Fungal*
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Genetic Complementation Test
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Hydro-Lyases / genetics*
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Hydro-Lyases / metabolism
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Molecular Sequence Data
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Open Reading Frames
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Phenotype
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Saccharomyces cerevisiae / genetics
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Sequence Analysis, DNA
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Sequence Analysis, Protein
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Sequence Homology, Amino Acid
Substances
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DNA, Fungal
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Fungal Proteins
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Hydro-Lyases
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imidazoleglycerolphosphate dehydratase