Structure of the mediator head module bound to the carboxy-terminal domain of RNA polymerase II

Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17931-5. doi: 10.1073/pnas.1215241109. Epub 2012 Oct 15.

Abstract

The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests a basis for regulation by phosphorylation of the CTD. Biochemical studies show a requirement for Mediator-CTD interaction for transcription.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Crystallography, X-Ray
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation
  • RNA Polymerase II / chemistry
  • RNA Polymerase II / metabolism*
  • Sequence Homology, Amino Acid
  • Tandem Mass Spectrometry

Substances

  • RNA Polymerase II

Associated data

  • PDB/4GWP
  • PDB/4GWQ