Abstract
CDDP is loaded into methoxypoly(ethylene glycol)-block-poly(L-glutamic acid) (mPEG-b-PLG), and a combination with iRGD is applied for NSCLC chemotherapy. The CDDP-loaded micelles show sustained cisplatin release in PBS, dose- and time-dependent inhibition to HeLa and A549 cell proliferation, and no apparent hemolysis activities. In in vivo studies using subcutaneous NSCLC xenograft models (A549), both free CDDP and CDDP-loaded micelles show an evident anti-tumor effect. However, the toxicity of CDDP is significantly reduced in the cases of CDDP-loaded micelles and co-administration with iRGD, and the survival time is prolonged by over 30%. Therefore, mPEG-b-PLG-loaded cisplatin and the combination with iRGD provides a promising new therapy for NSCLC.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antineoplastic Agents / chemistry
-
Antineoplastic Agents / pharmacokinetics
-
Antineoplastic Agents / pharmacology*
-
Carcinoma, Non-Small-Cell Lung / drug therapy*
-
Carcinoma, Non-Small-Cell Lung / mortality
-
Carcinoma, Non-Small-Cell Lung / pathology
-
Cell Line, Tumor
-
Cell Proliferation / drug effects
-
Cell Survival / drug effects
-
Cisplatin / chemistry
-
Cisplatin / pharmacokinetics
-
Cisplatin / pharmacology*
-
Drug Carriers / chemical synthesis*
-
Humans
-
Hydrogen-Ion Concentration
-
Lung Neoplasms / drug therapy*
-
Lung Neoplasms / mortality
-
Lung Neoplasms / pathology
-
Mice
-
Mice, Nude
-
Micelles
-
Microscopy, Electron, Transmission
-
Peptides, Cyclic / chemistry*
-
Polyethylene Glycols / chemical synthesis*
-
Polyglutamic Acid / analogs & derivatives*
-
Polyglutamic Acid / chemical synthesis
-
Survival Rate
-
Tumor Burden / drug effects
-
Xenograft Model Antitumor Assays
Substances
-
Antineoplastic Agents
-
Drug Carriers
-
Micelles
-
Peptides, Cyclic
-
cyclic arginine-glycine-aspartic acid peptide
-
methoxypoly(ethylene glycol)-block-poly(glutamic acid)
-
Polyglutamic Acid
-
Polyethylene Glycols
-
Cisplatin