Essential roles of PIKfyve and PTEN on phagosomal phosphatidylinositol 3-phosphate dynamics

FEBS Lett. 2012 Nov 16;586(22):4010-5. doi: 10.1016/j.febslet.2012.09.043. Epub 2012 Oct 12.

Abstract

PtdIns(3)P (phosphatidylinositol 3-phosphate) is a signaling molecule important for phagosome maturation. The major role of Vps34 in production of phagosomal PtdIns(3)P has been indicated. However, the fate of the newly generated PtdIns(3)P has not been well described. Here we show that elimination of PtdIns(3)P from phagosomal membrane was significantly delayed in RAW264.7 macrophages lacking PTEN or PIKfyve. In the PTEN-deficient cells treated with a PIKfyve inhibitor, degradation of PtdIns(3)P was almost lost, indicating that PTEN and PIKfyve are two major players in phagosomal PtdIns(3)P metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminopyridines / pharmacology
  • Animals
  • Cell Line
  • Female
  • Heterocyclic Compounds, 3-Ring / pharmacology
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Microscopy, Fluorescence
  • PTEN Phosphohydrolase / genetics
  • PTEN Phosphohydrolase / metabolism*
  • Phagosomes / metabolism*
  • Phosphatidylinositol 3-Kinases / genetics
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Phosphatidylinositol Phosphates / metabolism*
  • Phosphoinositide-3 Kinase Inhibitors
  • RNA Interference

Substances

  • 6-amino-N-(3-(4-(4-morpholinyl)pyrido(3',2'-4,5)furo(3,2-d)pyrimidin-2-yl)phenyl)-3-pyridinecarboxamide
  • Aminopyridines
  • Heterocyclic Compounds, 3-Ring
  • Phosphatidylinositol Phosphates
  • Phosphoinositide-3 Kinase Inhibitors
  • phosphatidylinositol 3-phosphate
  • Pikfyve protein, mouse
  • PTEN Phosphohydrolase
  • Pten protein, mouse