Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp

Monia Vitullo; Kathie Ann Grant; Michela Lucia Sammarco; Manuela Tamburro; Giancarlo Ripabelli; Corinne Francoise Laurence Amar

doi:10.1016/j.mcp.2012.10.001

Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp

Mol Cell Probes. 2013 Feb;27(1):68-70. doi: 10.1016/j.mcp.2012.10.001. Epub 2012 Oct 9.

Authors

Monia Vitullo¹, Kathie Ann Grant, Michela Lucia Sammarco, Manuela Tamburro, Giancarlo Ripabelli, Corinne Francoise Laurence Amar

Affiliation

¹ Department of Medicine and of Health Sciences, University of Molise, Campobasso, Italy.

PMID: 23064121
DOI: 10.1016/j.mcp.2012.10.001

Abstract

A 5'-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L. monocytogenes.

MeSH terms

Bacterial Proteins / genetics
Bacterial Typing Techniques / methods*
DNA, Bacterial / analysis
Food Microbiology
Listeria monocytogenes / classification*
Listeria monocytogenes / genetics*
Listeria monocytogenes / isolation & purification
Real-Time Polymerase Chain Reaction / methods*
Serotyping / methods
Species Specificity

Substances

Bacterial Proteins
DNA, Bacterial