Molecular mechanisms of fMLP-induced superoxide generation and degranulation in mouse neutrophils

Adv Biol Regul. 2013 Jan;53(1):128-34. doi: 10.1016/j.jbior.2012.09.001. Epub 2012 Sep 28.

Abstract

In this manuscript, involvement of PLD in fMLP-induced superoxide generation and degranulation were re-investigated using PLD(-/-) neutrophils, and the molecular mechanisms of these neutrophil functions were examined. Neither PLD1 nor PLD2 is involved in these fMLP-induced neutrophil functions. The results obtained in this study provide evidence that cPKC plays an important role in fMLP-induced superoxide generation. On the other hand, Ca(2+)-dependent signaling pathway and cPKC seem to be involved in degranulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism
  • Calcium Ionophores / pharmacology
  • Cell Degranulation / drug effects*
  • Diglycerides / pharmacology
  • Mice
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology*
  • Neutrophils / cytology
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • Phosphatidylcholines / metabolism
  • Phospholipase D / metabolism
  • Protein Kinase C / metabolism*
  • Signal Transduction
  • Superoxides / metabolism*

Substances

  • Calcium Ionophores
  • Diglycerides
  • Phosphatidylcholines
  • Superoxides
  • N-Formylmethionine Leucyl-Phenylalanine
  • 1-oleoyl-2-acetylglycerol
  • Protein Kinase C
  • phospholipase D2
  • Phospholipase D
  • phospholipase D1
  • Calcium