Abstract
In this manuscript, involvement of PLD in fMLP-induced superoxide generation and degranulation were re-investigated using PLD(-/-) neutrophils, and the molecular mechanisms of these neutrophil functions were examined. Neither PLD1 nor PLD2 is involved in these fMLP-induced neutrophil functions. The results obtained in this study provide evidence that cPKC plays an important role in fMLP-induced superoxide generation. On the other hand, Ca(2+)-dependent signaling pathway and cPKC seem to be involved in degranulation.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium / metabolism
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Calcium Ionophores / pharmacology
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Cell Degranulation / drug effects*
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Diglycerides / pharmacology
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Mice
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology*
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Neutrophils / cytology
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Neutrophils / drug effects*
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Neutrophils / metabolism
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Phosphatidylcholines / metabolism
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Phospholipase D / metabolism
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Protein Kinase C / metabolism*
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Signal Transduction
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Superoxides / metabolism*
Substances
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Calcium Ionophores
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Diglycerides
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Phosphatidylcholines
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Superoxides
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N-Formylmethionine Leucyl-Phenylalanine
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1-oleoyl-2-acetylglycerol
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Protein Kinase C
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phospholipase D2
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Phospholipase D
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phospholipase D1
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Calcium