In the search for novel antimicrobial agents, a series of new derivatives - N-substituted imides were prepared. All of the compounds were characterized by 'H NMR and ESI MS spectra. These derivatives were tested for antimicrobial activity. Microorganisms used in this study included aerobic and facultative anaerobic bacteria such as Staphylococcus aureus, Escherichia coli, Stenotrophomonas maltophilia, and obligatory anaerobes such as Bacteroides fragilis, Bacteroides thetaiotaomicron and Propionibacterium acnes. Moreover, Candida albicans yeast was used. For representatives of all species the MICs of the investigated compounds were determined. Most of investigated derivatives had no antimicrobial activity (MIC > 512 mg/L) except the derivative 22 which showed slight activity against Gram-positive aerobes and anaerobes.