Abstract
A new ingenane-type diterpene, (3S,5R)5-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-O-deoxyingenol (1), and six known compounds,3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-O-deoxyingenol (2), 20-O-decanoylingenol (3), 20-O-acetylingenol-3-O-(2'E,4'Z) decadienoate (4), kansuiphorin A (5), 3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoylingenol (6), and kansuinin F (7) were isolated and evaluated for their effect on IFN-γ production in NK92 cells. Interestingly, subjection to compounds 4 and 6 (10 nM) displayed the most significant response in IFN-γ production, comparable to that produced by the same dose of phorbol 12-myistate 13-acetate (PMA). High doses of compounds 3 (100 nM), 1 (1. 25 μM) and 5 (5.0 μM) have also been shown to activate the IFN-γ production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Allergic Agents / chemistry
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Anti-Allergic Agents / isolation & purification
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Anti-Allergic Agents / pharmacology
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Cell Line
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Cell Survival / drug effects
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Chromatography, High Pressure Liquid
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Diterpenes / chemistry*
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Diterpenes / isolation & purification
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Diterpenes / pharmacology*
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Drug Discovery
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Euphorbia / chemistry*
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Humans
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Immunologic Factors / chemistry
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Immunologic Factors / isolation & purification
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Immunologic Factors / pharmacology
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Interferon-gamma / metabolism*
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Interferon-gamma Release Tests
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Killer Cells, Natural / drug effects*
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Killer Cells, Natural / metabolism
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Lymphocyte Activation / drug effects*
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Medicine, Korean Traditional
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Osmolar Concentration
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Roots / chemistry*
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Republic of Korea
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Structure-Activity Relationship
Substances
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Anti-Allergic Agents
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Antineoplastic Agents, Phytogenic
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Diterpenes
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IFNG protein, human
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Immunologic Factors
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Plant Extracts
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ingenane
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Interferon-gamma