This paper investigates the removal of isopropanol by gas-phase biotrickling filtration. Two plastic packing materials, one structured and one random, have been evaluated in terms of oxygen mass transfer and isopropanol removal efficiency. Oxygen mass transfer experiments were performed at gas velocities of 104 and 312 m h⁻¹ and liquid velocities between 3 and 33 m h⁻¹. Both materials showed similar mass transfer coefficients up to liquid velocities of 15 m h⁻¹. At greater liquid velocities, the structured packing exhibited greater oxygen mass transfer coefficients. Biotrickling filtration experiments were carried out at inlet loads (IL) from 20 to 65 g C m⁻³ h⁻¹ and empty bed residence times (EBRT) from 14 to 160 s. To simulate typical industrial emissions, intermittent isopropanol loading (16 h/day, 5 day/week) and intermittent spraying frequency (15 min/1.5 h) were applied. Maximum elimination capacity of 51 g C m⁻³ h⁻¹ has been obtained for the random packing (IL of 65 g C m⁻³ h⁻¹, EBRT of 50 s). The decrease in irrigation frequency to 15 min every 3 h caused a decrease in the outlet emissions from 86 to 59 mg C Nm⁻³ (inlet of 500 mg C Nm⁻³). The expansion of spraying to night and weekend periods promoted the degradation of the isopropanol accumulated in the water tank during the day, reaching effluent concentrations as low as 44 mg C Nm⁻³. After a 7-week starvation period, the performance was recovered in less than 10 days, proving the robustness of the process.