Tissue specific delivery of estrone-conjugated siRNAs

Mol Biosyst. 2013 May;9(5):974-7. doi: 10.1039/c2mb25258a.

Abstract

An estrone phosphoramidite, synthesized in a single step, was directly incorporated at the 5' ends of small interfering RNAs (siRNAs). The resulting estrone-conjugated siRNAs readily pass through cellular membranes and down-regulate the target protein, and show specific distributions in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Estrone / chemistry*
  • Fluorescein-5-isothiocyanate / chemistry
  • Fluorescein-5-isothiocyanate / pharmacokinetics
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / pharmacokinetics
  • Gene Expression Profiling
  • Gene Knockdown Techniques / methods
  • Gene Transfer Techniques*
  • Humans
  • Lamin Type A / genetics
  • Lamin Type A / metabolism
  • MCF-7 Cells
  • Mice
  • Mice, Inbred ICR
  • Microscopy, Fluorescence
  • Models, Chemical
  • Molecular Structure
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / pharmacokinetics
  • Organophosphorus Compounds / chemical synthesis
  • Organophosphorus Compounds / chemistry*
  • RNA Interference
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Fluorescent Dyes
  • Lamin Type A
  • Oligodeoxyribonucleotides
  • Organophosphorus Compounds
  • RNA, Small Interfering
  • Vascular Endothelial Growth Factor A
  • phosphoramidite
  • Estrone
  • Fluorescein-5-isothiocyanate