Abstract
An estrone phosphoramidite, synthesized in a single step, was directly incorporated at the 5' ends of small interfering RNAs (siRNAs). The resulting estrone-conjugated siRNAs readily pass through cellular membranes and down-regulate the target protein, and show specific distributions in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Estrone / chemistry*
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Fluorescein-5-isothiocyanate / chemistry
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Fluorescein-5-isothiocyanate / pharmacokinetics
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacokinetics
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Gene Expression Profiling
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Gene Knockdown Techniques / methods
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Gene Transfer Techniques*
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Humans
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Lamin Type A / genetics
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Lamin Type A / metabolism
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MCF-7 Cells
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Mice
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Mice, Inbred ICR
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Microscopy, Fluorescence
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Models, Chemical
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Molecular Structure
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / pharmacokinetics
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Organophosphorus Compounds / chemical synthesis
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Organophosphorus Compounds / chemistry*
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RNA Interference
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RNA, Small Interfering / chemistry
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RNA, Small Interfering / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Tissue Distribution
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Vascular Endothelial Growth Factor A / genetics
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Vascular Endothelial Growth Factor A / metabolism
Substances
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Fluorescent Dyes
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Lamin Type A
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Oligodeoxyribonucleotides
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Organophosphorus Compounds
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RNA, Small Interfering
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Vascular Endothelial Growth Factor A
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phosphoramidite
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Estrone
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Fluorescein-5-isothiocyanate