Abstract
Based on enzyme-regulated unmodified gold nanoparticle aggregation, a visual and homogeneous assay of adenosine deaminase (ADA) activity without any other coupling enzymes, aptamers or additional modifications has been developed. The present strategy is simple, cost-effective, high throughput, selective and sensitive for ADA with a detection limit of 0.8227 U L(-1).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Deaminase / chemistry
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Adenosine Deaminase / metabolism*
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Biosensing Techniques / methods*
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Colorimetry
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Enzyme Assays* / economics
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Enzyme Inhibitors / chemistry
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Gold / chemistry*
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Limit of Detection
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Metal Nanoparticles / chemistry*
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Molecular Structure
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Time Factors
Substances
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Enzyme Inhibitors
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Gold
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Adenosine Deaminase