Enzyme-regulated unmodified gold nanoparticle aggregation: a label free colorimetric assay for rapid and sensitive detection of adenosine deaminase activity and inhibition

Chem Commun (Camb). 2012 Nov 18;48(89):10996-8. doi: 10.1039/c2cc36240f.

Abstract

Based on enzyme-regulated unmodified gold nanoparticle aggregation, a visual and homogeneous assay of adenosine deaminase (ADA) activity without any other coupling enzymes, aptamers or additional modifications has been developed. The present strategy is simple, cost-effective, high throughput, selective and sensitive for ADA with a detection limit of 0.8227 U L(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / chemistry
  • Adenosine Deaminase / metabolism*
  • Biosensing Techniques / methods*
  • Colorimetry
  • Enzyme Assays* / economics
  • Enzyme Inhibitors / chemistry
  • Gold / chemistry*
  • Limit of Detection
  • Metal Nanoparticles / chemistry*
  • Molecular Structure
  • Time Factors

Substances

  • Enzyme Inhibitors
  • Gold
  • Adenosine Deaminase