Purpose: To compare the conventional localized point-resolved spectroscopy (PRESS) with localized 2D intermolecular single-quantum coherence (iSQC) magnetic resonance spectroscopy (MRS) and obtain in vivo MRS spectrum of rat brain using the latter technique.
Materials and methods: A brain phantom, an intact pig brain tissue, and mature Sprague-Dawley rat were studied by PRESS, Nano magic-angle spinning spectroscopy, and iSQC MRS.
Results: Using PRESS, high-resolution MRS can be obtained from the brain phantom and pig brain tissue with a small voxel in a relatively homogeneous field. When a large voxel is selected, the field homogeneity is distinctly reduced. No useful information is obtained from the PRESS spectra. However, using the iSQC MRS, high-resolution spectra can be obtained from the two samples with a relatively large voxel. In the same way, an iSQC MRS spectrum can be obtained from a relatively large voxel of in vivo rat brain with a comparable resolution to the PRESS spectrum with a small voxel.
Conclusion: Compared to PRESS, the iSQC MRS may be more feasible and promising for detection of strongly structured tissues with relatively large voxels.
Copyright © 2012 Wiley Periodicals, Inc.