Corn silage (CS) is associated with a reduction in milk fat content. The fact that CS is constituted of a grain and a forage fraction could explain this effect. This experiment evaluated the effect of grain fraction of CS on rumen fermentation, production performance and milk composition. Earless CS (ECS) was harvested after manually removing corn ears from the plant. Whole CS (WCS) was harvested from the same field on the same day. Eight (four ruminally fistulated) multiparous Holstein cows (84 days in milk) were utilized in a double 4 × 4 Latin square with 21-day periods. Treatments were (dry matter (DM) basis) (1) 23.0% WCS; (2) 12.4% ECS plus 10.6% high moisture corn (HMC) to obtain reconstituted CS (RCS); (3) 23.0% ECS; and (4) 23.0% timothy silage (TS). Diets were formulated to be isonitrogenous and were fed as total mixed ration once a day. DM intake (DMI), milk yield, 4.0% fat-corrected milk (FCM), as well as protein concentration and yield were higher for WCS than ECS. Compared with WCS, cows tended to eat less with RCS, and produced less milk and milk protein. However, yield of FCM was similar between WCS and RCS. Milk fat concentration and yield, as well as the specific ratio of t11 18:1 to t10 18:1 in milk fat did not differ among diets. Milk urea-N tended to be higher for ECS than WCS and TS, whereas ruminal NH3-N was higher with ECS than TS. Rumen pH decreased linearly with time after feeding but was not different between treatments. Higher acetate and lower propionate concentration resulted in greater acetate to propionate ratio with ECS compared with WCS. In conclusion, removing grain fraction from CS decreased milk production and modified rumen fermentation without affecting milk fat concentration and yield. Moreover, despite some differences in DMI and total ruminal volatile fatty acid concentration between WCS and RCS, the restoration of FCM yield, using HMC in RCS diets, to a level of production similar to WCS highlights the importance of energy and nutrients supplied by the grain fraction of CS to support milk yield.