Objective: To optimize the preparation procedure for extraction of flavonoids from leaves of Heritiera littoralis.
Methods: Comparing microwave extraction and ultrasonic extraction, The content of total flavonoids was measure by UV spectrophometry and the wavelength set at 510 nm. The effects of treatment time, extraction power, ethanol concentration and solid-liquid ratio were studied. The orthogonal test of four factors and three levels were studied to obtain the extraction technology of flavonoids from leaves of Heritiera littoralis.
Results: The optimal technical conditions were mixing leaves of Heritiera littoralis with 70% ethanol on the ratio of 1 g: 30 mL, when microwave treatment time was 8 min and micro-wave power was 400 W. Under this condition, the extraction rate reached 6.15%.
Conclusion: This study explores a practical extraction method for flavonoids from leaves of Heritiera littoralis, and lay a good foundation for mangrove medical research in future.