A new strategy for effective construction of protein stacks by using cucurbit[8]uril as a glue molecule

Chem Commun (Camb). 2012 Nov 7;48(86):10633-5. doi: 10.1039/c2cc33380e.

Abstract

Cucurbit[8]uril (CB[8]) is found to induce the aggregation of pristine proteins in aqueous solution. Based on this finding, a new strategy for effective construction of layer-by-layer homo- and hetero-protein stacks was developed, where their bioactivities are preserved.

MeSH terms

  • Animals
  • Bridged-Ring Compounds / chemistry*
  • Catalase / metabolism
  • Cattle
  • Hemoglobins / chemistry
  • Hemoglobins / metabolism
  • Hydrogen Peroxide / chemistry
  • Imidazoles / chemistry*
  • Proteins / chemistry*
  • Proteins / metabolism
  • Spectrophotometry, Ultraviolet
  • Water / chemistry

Substances

  • Bridged-Ring Compounds
  • Hemoglobins
  • Imidazoles
  • Proteins
  • cucurbit(8)uril
  • Water
  • Hydrogen Peroxide
  • Catalase