Toxin detection in patients' sera by mass spectrometry during two outbreaks of type A Botulism in France

J Clin Microbiol. 2012 Dec;50(12):4091-4. doi: 10.1128/JCM.02392-12. Epub 2012 Sep 19.

Abstract

In two outbreaks of food-borne botulism in France, Clostridium botulinum type A was isolated and characterized from incriminated foods. Botulinum neurotoxin type A was detected in the patients' sera by mouse bioassay and in vitro endopeptidase assay with an immunocapture step and identification of the cleavage products by mass spectrometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Assay / methods
  • Botulinum Toxins, Type A / blood*
  • Botulism / diagnosis*
  • Botulism / epidemiology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Disease Models, Animal
  • Disease Outbreaks*
  • Foodborne Diseases / diagnosis
  • Foodborne Diseases / epidemiology
  • France / epidemiology
  • Humans
  • Mass Spectrometry / methods*
  • Mice
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Serum / chemistry*

Substances

  • DNA, Bacterial
  • Botulinum Toxins, Type A

Associated data

  • GENBANK/JQ954969
  • GENBANK/JQ954970