Transfection of Plasmodium falciparum

Methods Mol Biol. 2013:923:75-98. doi: 10.1007/978-1-62703-026-7_6.

Abstract

Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques
  • DNA, Protozoan
  • Electroporation / instrumentation
  • Electroporation / methods
  • Erythrocytes / parasitology
  • Gene Order
  • Humans
  • Nucleic Acid Hybridization / methods
  • Plasmids / genetics
  • Plasmodium falciparum / drug effects
  • Plasmodium falciparum / genetics*
  • Plasmodium falciparum / growth & development
  • Polymerase Chain Reaction
  • Transfection / instrumentation
  • Transfection / methods*

Substances

  • DNA, Protozoan