Reduction of soybean meal non-starch polysaccharides and α-galactosides by solid-state fermentation using cellulolytic bacteria obtained from different environments

PLoS One. 2012;7(9):e44783. doi: 10.1371/journal.pone.0044783. Epub 2012 Sep 11.

Abstract

Soybean meal (SBM) is an important protein source in animal feed. However, the levels of SBM inclusion are restricted in some animal species by the presence of antinutritional factors (ANFs), including non-starch polysaccharides (NSPs) and α-galactosides (GOSs). The aim of this study was to reduce the soybean meal NSPs and GOSs by solid-state fermentation (SSF) using a combination of cellulolytic bacteria isolated from different environments (termites, earthworms, corn silage and bovine ruminal content). To analyse the key enzymatic activities, the isolates were grown in minimal media containing NSPs extracted from SBM. The selected bacterial strains belonged to the genera Streptomyces, Cohnella and Cellulosimicrobium. SSF resulted in a reduction of nearly 24% in the total NSPs, 83% of stachyose and 69% of raffinose and an increase in the protein content. These results suggest that cellulolytic bacteria-based SSF processing facilitates SBM nutritional improvement. In addition, the use of fermented SBM in animal diets can be recommended.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed / analysis*
  • Animals
  • Bacteria / metabolism
  • Cellulase / chemistry
  • Cellulose / chemistry
  • DNA Primers / chemistry
  • Fermentation
  • Galactosides / chemistry
  • Glycine max / metabolism*
  • Oligosaccharides / chemistry
  • Polymorphism, Restriction Fragment Length
  • Polysaccharides, Bacterial / metabolism
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal, 16S / metabolism
  • Raffinose / chemistry
  • Rumen / metabolism
  • Starch / chemistry
  • Streptomyces / metabolism

Substances

  • DNA Primers
  • Galactosides
  • Oligosaccharides
  • Polysaccharides, Bacterial
  • RNA, Ribosomal
  • RNA, Ribosomal, 16S
  • stachyose
  • Cellulose
  • Starch
  • Cellulase
  • Raffinose

Grants and funding

This investigation was supported by grants FONDECYT 1080480/110253 from CONICYT-Chile and INNOVA CHILE 05CT6PPT-09 from CORFO. R. Opazo was supported by fellowships from CONICYT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.