Aim: To explore the effect of perilipin 1 on chicken preadipocyte lipid accumulation.
Methods: Firstly, pcDNA3.1-perilipin 1 gene eukaryotic expression vector was constructed and transfected into primary cultured chicken preadipocytes. Twenty-four hours after transfection, chicken preadipocytes were cultured in complete medium with oleate, to induce preadipocyte differentiation. Then perilipin 1 was detected by Western blotting; the role of perilipin 1 in preadipocyte lipid accumulation was investigated by Oil Red O staining extraction assay; the expression levels of other genes (FAS, ACC and ATGL) related to the chicken adipocyte lipid metabolism were tested by real-time RT-PCR.
Results: pcDNA3.1-perilipin 1 gene eukaryotic expression vector upregulated the expression of perilipin 1 in the transfected chicken preadipocytes. Over-expression of perilipin 1 promoted chicken preadipocyte lipid accumulation, while there was no significant change in the expression levels of other chicken lipid metabolism related genes (FAS, ACC and ATGL).
Conclusion: Over-expression of perilipin 1 can promote chicken preadipocyte lipid accumulation.