Inhibition of toxigenesis of group II (nonproteolytic) Clostridium botulinum type B in meat products by using a reduced level of nitrite

J Food Prot. 2012 Jul;75(7):1346-9. doi: 10.4315/0362-028X.JFP-12-056.

Abstract

The effect of three different concentrations of sodium nitrite (0, 75, and 120 mg/kg) on growth and toxigenesis of group II (nonproteolytic) Clostridium botulinum type B was studied in Finnish wiener-type sausage, bologna-type sausage, and cooked ham. A low level of inoculum (2.0 log CFU/g) was used for wiener-type sausage and bologna-type sausage, and both low (2.0 log CFU/g) and high (4.0 log CFU/g) levels were used for cooked ham. The products were formulated and processed under simulated commercial conditions and stored at 8°C for 5 weeks. C. botulinum counts were determined in five replicate samples of each nitrite concentration at 1, 3, and 5 weeks after thermal processing. All samples were positive for C. botulinum type B. The highest C. botulinum counts were detected in nitrite-free products. Toxigenesis was observed in nitrite-free products during storage, but products containing either 75 or 120 mg/kg nitrite remained nontoxic during the 5-week study period, suggesting that spores surviving the heat treatment were unable to germinate and develop into a toxic culture in the presence of nitrite. The results suggest that the safety of processed meat products with respect to group II C. botulinum type B can be maintained even with a reduced concentration (75 mg/kg) of sodium nitrite.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Botulinum Toxins / analysis
  • Clostridium botulinum type B / drug effects*
  • Clostridium botulinum type B / physiology
  • Colony Count, Microbial
  • Consumer Product Safety
  • Dose-Response Relationship, Drug
  • Food Handling / methods*
  • Hot Temperature
  • Humans
  • Meat Products / microbiology*
  • Nitrites / pharmacology*
  • Spores, Bacterial / drug effects
  • Spores, Bacterial / growth & development
  • Time Factors

Substances

  • Nitrites
  • Botulinum Toxins