This paper presents both a theoretical and an experimental investigation of the influence of the mean flow on the input impedance of a duct. The input impedance of an axisymetrical flow duct is calculated, taking into account the convective effect of a uniform flow, the dissipative effect of a turbulent flow and the radiation in an open jet. Each of these effects is separately studied. An experimental apparatus has been specifically designed to lower flow noise on the transducers, taking advantage of the Two-Microphone-Three-Calibration (TMTC) method [V. Gibiat and F. Laloë, J. Acoust. Soc. Am. 88, 2533-2545 (1990)], whose full calibration process allows any geometry for the measurement head. Theory and experiments are compared for a 1 m long cylindrical duct carrying a flow whose Mach number equals up to 0.15. The resonant frequencies are in close agreement, within 3%. The relative evolution of the magnitude maxima with increasing flow are in good agreement, within 10%. Despite similar tendencies when modifying the mean flow velocity, the amplitude of variation of the magnitude is 2 to 5 times smaller in the experiments.