Novel primer-probe sets for detection and identification of mycobacteria by PCR-microarray assay

J Clin Microbiol. 2012 Nov;50(11):3777-9. doi: 10.1128/JCM.02300-12. Epub 2012 Sep 12.

Abstract

A PCR-microarray assay was developed in which PCR primers and hybridization probes were designed for the 16S rRNA genes of 16 clinically relevant mycobacteria and for IS6110 of Mycobacterium tuberculosis. The assay, based on a multiplex PCR followed by hybridization with oligonucleotide probes, was tested against 16 Mycobacterium species and 70 clinical samples.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / methods*
  • DNA Primers / genetics
  • DNA Transposable Elements
  • Humans
  • Microarray Analysis / methods*
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Mycobacterium tuberculosis / classification*
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification*
  • Oligonucleotide Probes / genetics
  • RNA, Ribosomal, 16S / genetics
  • Tuberculosis / diagnosis*
  • Tuberculosis / microbiology

Substances

  • DNA Primers
  • DNA Transposable Elements
  • Oligonucleotide Probes
  • RNA, Ribosomal, 16S