Probing lymphoma infiltration in spleen of AKR/J mice chronically exposed to electromagnetic fields for risk assessment--toward noninvasive modeling

Abstract

Using the AKR/J mouse model, the potential of Raman spectroscopy for monitoring lymphoma in predisposed subjects is demonstrated by discriminating lymphoma infiltration in spleens; the relevance of different excitation profiles is shown. Under green excitation with optimal fluorescence bleaching, stronger DNA bands, intensity variations at amide-III and phenylalanine bands, and the behavior of the 1606/1639 cm(-1) doublet correlate with tumorigenesis. Under red excitation, Raman fingerprints with multivariate models help to discriminate AKR/J-mouse histological subtypes: Lymphoblastic lymphoma (LB) is found significantly distant from both separated lymphocytic lymphoma (LL) and healthy spleen; this agrees with histology since LB has well differentiated large lymphoma cells, while LL, with smaller cells similar to normal lymphocytes, usually cannot be discriminated from normal tissue without histoimmunoassays.

Keywords: Cancer; Raman spectroscopy; noninvasive detection; radiation risk.