A strategy to enhance the binding affinity of fluorophore-aptamer pairs for RNA tagging with neomycin conjugation

Jongho Jeon; Kyung Hyun Lee; Jianghong Rao

doi:10.1039/c2cc34498j

A strategy to enhance the binding affinity of fluorophore-aptamer pairs for RNA tagging with neomycin conjugation

Chem Commun (Camb). 2012 Oct 14;48(80):10034-6. doi: 10.1039/c2cc34498j.

Authors

Jongho Jeon¹, Kyung Hyun Lee, Jianghong Rao

Affiliation

¹ Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Stanford, California 94305-5484, USA.

Abstract

Fluorogenic sulforhodamine-neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250-400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Binding Sites
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Neomycin / chemical synthesis
Neomycin / chemistry*
Rhodamines / chemical synthesis
Rhodamines / chemistry*

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Rhodamines
Neomycin

Abstract

Publication types

MeSH terms

Substances

Grants and funding