Synthesis of triptorelin lactate catalyzed by lipase in organic media

Hong Zhuang; Zhi Wang; Jiaxin Wang; Hong Zhang; Erna Xun; Ge Chen; Hong Yue; Ning Tang; Lei Wang

doi:10.3390/ijms13089971

Synthesis of triptorelin lactate catalyzed by lipase in organic media

Int J Mol Sci. 2012;13(8):9971-9979. doi: 10.3390/ijms13089971. Epub 2012 Aug 10.

Authors

Hong Zhuang¹, Zhi Wang², Jiaxin Wang², Hong Zhang^{2

3}, Erna Xun², Ge Chen², Hong Yue², Ning Tang¹, Lei Wang²

Affiliations

¹ Department of Food Science and Engineering, Jilin University, Changchun 130021, China.
² Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130021, China.
³ College of Chemistry, Jilin University, Changchun 130023, China.

Abstract

Triptorelin lactate was successfully synthesized by porcine pancreatic lipase (PPL) in organic solvents. The effects of acyl donor, substrate ratio, organic solvent, temperature, and water activity were investigated. Under the optimum conditions, a yield of 30% for its ester could be achieved in the reaction for about 48 h.

Keywords: enzyme activity; esterification; lipase; triptorelin lactate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteria / enzymology
Catalysis
Esterification
Hydrogen-Ion Concentration
Kinetics
Lactates / chemistry*
Lipase / metabolism*
Organic Chemicals / chemistry*
Solvents / chemistry*
Swine
Temperature
Triptorelin Pamoate / analogs & derivatives*
Triptorelin Pamoate / chemical synthesis*
Triptorelin Pamoate / chemistry*
Water / chemistry*

Substances

Lactates
Organic Chemicals
Solvents
triptorelin lactate
Water
Triptorelin Pamoate
Lipase