Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme

Tian Tian; Heng Xiao; Yuelin Long; Xiaoe Zhang; Shaoru Wang; Xiang Zhou; Songmei Liu; Xin Zhou

doi:10.1039/c2cc35648a

Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme

Chem Commun (Camb). 2012 Oct 14;48(80):10031-3. doi: 10.1039/c2cc35648a.

Authors

Tian Tian¹, Heng Xiao, Yuelin Long, Xiaoe Zhang, Shaoru Wang, Xiang Zhou, Songmei Liu, Xin Zhou

Affiliation

¹ College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, PR China.

PMID: 22948177
DOI: 10.1039/c2cc35648a

Abstract

The analysis of DNA methylation and MTase (methyltransferase) activity is important in epigenetic study. We have developed a novel strategy for sensitive analysis of MTase activity based on a hairpin shaped DNAzyme; 8-17 DNAzyme amplicon has been adopted and found to be very effective in such analysis.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

DNA Methylation
DNA Probes / chemistry
DNA Probes / metabolism*
DNA, Catalytic / chemistry
DNA, Catalytic / metabolism*
Enzyme Assays / methods*
Sensitivity and Specificity
Site-Specific DNA-Methyltransferase (Adenine-Specific) / analysis*
Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism

Substances

DNA Probes
DNA, Catalytic
Dam methyltransferase
Site-Specific DNA-Methyltransferase (Adenine-Specific)