Injectable nanohybrid scaffold for biopharmaceuticals delivery and soft tissue engineering

Macromol Rapid Commun. 2012 Dec 13;33(23):2015-22. doi: 10.1002/marc.201200360. Epub 2012 Sep 3.

Abstract

An injectable nanofibrous hydrogel scaffold integrated with growth factors (GFs) loaded polysaccharide nanoparticles was developed that specifically allows for targeted adipose-derived stem cells (ASCs) encapsulation and soft tissue engineering. The nanofibrous hydrogel was produced via biological conjugation of biotin-terminated star-shaped poly(ethylene glycol) (PEG-Biotin) and streptavidin-functionalized hyaluronic acid (HA-Streptavidin). The polysaccharide nanoparticles were noncovalently assembled via electrostatic interactions between low-molecular-weight heparin (LMWH) and N,N,N-trimethylchitosan chloride (TMC). Vascular endothelial growth factor (VEGF) was entrapped in the LMWH/TMC nanoparticles by affinity interactions with LMWH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsules
  • Chitosan / chemistry
  • Drug Carriers / chemistry
  • Drug Delivery Systems / methods*
  • Heparin, Low-Molecular-Weight / chemistry
  • Humans
  • Hyaluronic Acid / chemistry
  • Hydrogels / chemistry*
  • Injections
  • Nanofibers / chemistry*
  • Polyethylene Glycols / chemistry
  • Static Electricity
  • Stem Cells / cytology
  • Tissue Engineering / methods*
  • Vascular Endothelial Growth Factor A / administration & dosage
  • Vascular Endothelial Growth Factor A / chemistry

Substances

  • Capsules
  • Drug Carriers
  • Heparin, Low-Molecular-Weight
  • Hydrogels
  • N-trimethyl chitosan chloride
  • Vascular Endothelial Growth Factor A
  • Polyethylene Glycols
  • Hyaluronic Acid
  • Chitosan