Enantioselective oxidation of aldehydes catalyzed by alcohol dehydrogenase

Paul Könst; Hedda Merkens; Selin Kara; Svenja Kochius; Andreas Vogel; Ralf Zuhse; Dirk Holtmann; Isabel W C E Arends; Frank Hollmann

doi:10.1002/anie.201203219

Enantioselective oxidation of aldehydes catalyzed by alcohol dehydrogenase

Angew Chem Int Ed Engl. 2012 Sep 24;51(39):9914-7. doi: 10.1002/anie.201203219. Epub 2012 Aug 31.

Authors

Paul Könst¹, Hedda Merkens, Selin Kara, Svenja Kochius, Andreas Vogel, Ralf Zuhse, Dirk Holtmann, Isabel W C E Arends, Frank Hollmann

Affiliation

¹ Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.

PMID: 22936647
DOI: 10.1002/anie.201203219

Abstract

Teaching old dogs new tricks: Alcohol dehydrogenases (ADHs) may be established redox biocatalysts but they still are good for a few surprises. ADHs can be used to oxidize aldehydes, and this was demonstrated by the oxidative dynamic kinetic resolution of profens. In the presence of a suitable cofactor regeneration system, this reaction can occur with high selectivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Dehydrogenase / genetics
Alcohol Dehydrogenase / metabolism*
Alcohols / metabolism
Aldehydes / metabolism*
Escherichia coli / enzymology*
Escherichia coli / genetics
Kinetics
Lactobacillus / enzymology
Oxidation-Reduction
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Stereoisomerism

Substances

Alcohols
Aldehydes
Recombinant Proteins
Alcohol Dehydrogenase