Abstract
Centrioles are duplicated during S-phase to generate the two centrosomes that serve as mitotic spindle poles during mitosis. The centrosomal pool of the Mps1 kinase is important for centriole assembly, but how Mps1 is delivered to centrosomes is unknown. Here we have identified a centrosome localization domain within Mps1 and identified the mitochondrial porin VDAC3 as a protein that binds to this region of Mps1. Moreover, we show that VDAC3 is present at the mother centriole and modulates centriole assembly by recruiting Mps1 to centrosomes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / metabolism*
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Centrioles / metabolism*
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Green Fluorescent Proteins / metabolism
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HEK293 Cells
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HeLa Cells
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Humans
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Mice
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Mitochondrial Membrane Transport Proteins / metabolism*
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Molecular Sequence Data
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NIH 3T3 Cells
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Protein Binding
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / metabolism*
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Protein Transport
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Protein-Tyrosine Kinases / chemistry
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Protein-Tyrosine Kinases / metabolism*
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RNA, Small Interfering / metabolism
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Voltage-Dependent Anion Channels / metabolism*
Substances
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Cell Cycle Proteins
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Mitochondrial Membrane Transport Proteins
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RNA, Small Interfering
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VDAC3 protein, human
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Voltage-Dependent Anion Channels
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Green Fluorescent Proteins
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Protein-Tyrosine Kinases
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Protein Serine-Threonine Kinases
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TTK protein, human