Development of microsatellite markers for Anadenanthera colubrina var. cebil (Fabaceae), a native tree from South America

Am J Bot. 2012 Sep;99(9):e372-4. doi: 10.3732/ajb.1200078. Epub 2012 Aug 30.

Abstract

Premise of the study: Microsatellite primers were developed in the native legume tree Anadenanthera colubrina var. cebil to study the genetic diversity and genetic structure in natural populations in Argentina.

Methods and results: Nine microsatellite markers were identified using a genomic library enriched for tandemly repeated motifs, eight of which markers were polymorphic. The polymorphism of these markers was assessed by investigating 20 individuals for fragment polymorphism; three to 13 alleles were observed for each locus. Observed and expected heterozygosities ranged from 0.300 to 1.000 and from 0.463 to 0.900, respectively.

Conclusions: These results confirm that these primers will be useful for investigating the genetic diversity and genetic structure of natural populations of A. colubrina var. cebil in future studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Argentina
  • DNA Primers / genetics
  • Fabaceae / genetics*
  • Genetic Loci / genetics
  • Microsatellite Repeats / genetics*
  • Molecular Sequence Data
  • Trees / genetics*

Substances

  • DNA Primers

Associated data

  • GENBANK/JQ086537
  • GENBANK/JQ086538
  • GENBANK/JQ086539
  • GENBANK/JQ086540
  • GENBANK/JQ086541
  • GENBANK/JQ086542
  • GENBANK/JQ086543
  • GENBANK/JQ086544
  • GENBANK/JQ806379