Modification of one epitope-flanking amino acid allows for the induction of friend retrovirus-specific CD8+ T cells by Adenovirus-based immunization

J Virol. 2012 Nov;86(22):12422-5. doi: 10.1128/JVI.01607-12. Epub 2012 Aug 29.

Abstract

While Friend retrovirus-infected mice readily mount a vigorous CD8(+) T cell response to the leader-gag-derived peptide GagL(85-93), no GagL(85-93)-specific T cells were detectable in mice immunized against Friend virus (FV) with viral vectors or DNA vaccines. By exchanging one epitope-flanking amino acid or using a scaffold protein we were able to demonstrate for the first time the induction of GagL(85-93)-specific CD8(+) T cells by genetic vaccination and show their high protective effect against FV challenge infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Vaccines / immunology*
  • Animals
  • CD8-Positive T-Lymphocytes / virology*
  • Capsid / chemistry
  • Epitopes / chemistry*
  • Friend murine leukemia virus / metabolism
  • Gene Products, gag / chemistry
  • Immunization
  • Mice
  • Models, Genetic
  • Peptides / chemistry
  • Retroviridae / metabolism*
  • Vaccination
  • Vaccines, DNA / chemistry
  • Viral Vaccines / metabolism

Substances

  • Adenovirus Vaccines
  • Epitopes
  • Gene Products, gag
  • Peptides
  • Vaccines, DNA
  • Viral Vaccines