Abstract
A copolymer system of 2-aminoethyl methacrylate and N-isopropylacrylamide comprises novel properties: changes in conformation and hydrophilicity upon heating influence the antibacterial activity and result in a switchable biocidal effect. The copolymers are characterized via NMR, MALDI-ToF MS, phase transition behavior, and antibacterial tests with E. coli or B. subtilis. MIC and MBC are determined using standard dilution methods, temperature-dependence via incubation at different temperatures and cytotoxicity by MTT tests. The copolymers exhibit lower MIC in globule than coil conformation, crosslinking on cotton results in non-leaching materials with better antibacterial activity above than below the phase-transition point.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / chemical synthesis*
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Anti-Bacterial Agents / pharmacology
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Bacillus subtilis / drug effects*
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Bacillus subtilis / growth & development
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Biocompatible Materials / chemical synthesis*
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Biocompatible Materials / pharmacology
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Cell Line
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Cell Survival / drug effects
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Escherichia coli / drug effects*
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Escherichia coli / growth & development
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Hydrophobic and Hydrophilic Interactions
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Magnetic Resonance Spectroscopy
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Mice
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Microbial Sensitivity Tests
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Microbial Viability / drug effects
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Molecular Conformation
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Phase Transition
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Polymethacrylic Acids / chemical synthesis*
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Polymethacrylic Acids / pharmacology
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Temperature
Substances
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Anti-Bacterial Agents
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Biocompatible Materials
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Polymethacrylic Acids
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poly(2-aminoethyl methacrylate-co-N-isopropylacrylamide)