Abstract
Three isotypes of phosphoinositide-specific phospholipase C designated CcPLC1, CcPLC2, and CcPLC3 were identified in Coprinopsis cinerea, through a search of the genome sequence database. The functional role of the PI-PLCs were studied by using U73122, which specifically inhibits the activity of PI-PLC. The specificity of the inhibitor effect was confirmed by using an inactive structural analog U73433. The inhibition of PI-PLCs activity resulted in severely retarded germination of basidiospores and oidia, reduced hyphal growth, knobbly hyphal tips with many irregular side branches, and aberrant (branch-like structure) clamp cells. Furthermore, U73122 definitely inhibited cell wall formation. Here we report that PI-PLCs play important roles in various aspects of C. cinerea biology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agaricales / drug effects
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Agaricales / enzymology*
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Agaricales / genetics
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Agaricales / growth & development*
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Cell Wall / metabolism*
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Computational Biology / methods
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Enzyme Inhibitors / metabolism
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Estrenes / metabolism
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Genome, Fungal
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Hyphae / drug effects
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Hyphae / enzymology
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Hyphae / growth & development
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Phosphatidylinositols / metabolism*
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Pyrrolidinones / metabolism
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Regeneration
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Spores, Fungal / drug effects
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Spores, Fungal / enzymology
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Spores, Fungal / growth & development
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Type C Phospholipases / antagonists & inhibitors
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Type C Phospholipases / genetics
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Type C Phospholipases / metabolism*
Substances
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Enzyme Inhibitors
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Estrenes
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Phosphatidylinositols
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Pyrrolidinones
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1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
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Type C Phospholipases