Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms

Caries Res. 2012;46(6):549-54. doi: 10.1159/000341190. Epub 2012 Aug 22.

Abstract

Aim: The effect of photodynamic antimicrobial chemotherapy (PACT) on oral biofilms was evaluated.

Methods: Biofilms formed in vitro were treated with sensitizer (S) and/or light (L) in the test (S+L+) and control (S-L-, S+L-, S-L+) groups. Additionally, 21 volunteers wore intraoral devices and the biofilms formed on each side of the device were referred to as S+L+ or S-L- groups.

Results: Significant decreases in the viability of in vitro biofilms were observed after PACT. PACT was ineffective in inhibiting multi-species biofilms formed in situ.

Conclusion: PACT was effective on in vitro biofilms and was ineffective on in situ biofilms.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use*
  • Biofilms / drug effects*
  • Dental Caries / drug therapy*
  • Dental Caries / microbiology
  • Female
  • Humans
  • Male
  • Microbial Viability / drug effects
  • Photochemotherapy*
  • Photosensitizing Agents / pharmacology*
  • Single-Blind Method
  • Statistics, Nonparametric
  • Streptococcus mutans / drug effects*
  • Tolonium Chloride / pharmacology*
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Photosensitizing Agents
  • Tolonium Chloride