Simultaneous measurement of adenosine, dopamine, acetylcholine and 5-hydroxytryptamine in cerebral mice microdialysis samples by LC-ESI-MS/MS

J Pharm Biomed Anal. 2012 Dec:71:183-6. doi: 10.1016/j.jpba.2012.08.004. Epub 2012 Aug 10.

Abstract

A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous measurement of adenosine (ADE), dopamine (DA), acetylcholine (ACh) and 5-hydroxytryptamine (5-HT) in mouse brain microdialysates. High method sensitivity (LLOQ of 0.05nM) was achieved by optimization of chromatographic and mass spectrometric parameters. The method was fully validated for its sensitivity, selectivity, matrix effect and stability. The LC-MS/MS method was successfully applied to evaluate the effect of the systemic administration of cocaine or amphetamine on the extracellular levels of ADE, DA, ACh and 5-HT in the mouse nucleus accumbens by microdialysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / analysis*
  • Adenosine / analysis*
  • Amphetamine / chemistry
  • Amphetamine / pharmacology
  • Animals
  • Chromatography, Liquid / methods*
  • Cocaine / chemistry
  • Cocaine / pharmacology
  • Dopamine / analysis*
  • Mice
  • Microdialysis / methods
  • Nucleus Accumbens / chemistry*
  • Nucleus Accumbens / drug effects
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serotonin / analysis*
  • Tandem Mass Spectrometry / methods*

Substances

  • Serotonin
  • Amphetamine
  • Cocaine
  • Adenosine
  • Acetylcholine
  • Dopamine