Abstract
Aeromonas hydrophila is an important fish pathogen responsible for huge economic losses in aquaculture sector. The bacterial outer membrane proteins (OMPs), especially adhesins play a key role in the virulence of the bacteria and are considered potential vaccine candidates. We evaluated the immunogenicity of two important outer membrane proteins namely Aha1 and OmpW of A. hydrophila. These proteins were over-expressed in Escherichia coli, purified and used for the vaccination of common carp. Sequence analysis predicted that, Aha1 and OmpW are adhesins and antigenic. Common carp immunized with recombinant Aha1 and OmpW proteins showed significant antibody production and a relative percentage survival of 52 and 71 respectively indicating their protective efficacy against A. hydrophila infection.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aeromonas hydrophila / genetics
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Aeromonas hydrophila / immunology*
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Animals
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Antibodies, Bacterial / immunology
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / immunology*
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Bacterial Vaccines / genetics
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Bacterial Vaccines / immunology
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Bacterial Vaccines / pharmacology*
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Carps*
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Cloning, Molecular
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Drugs, Chinese Herbal
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Fish Diseases / immunology
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Fish Diseases / microbiology*
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Fish Diseases / prevention & control*
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Gram-Negative Bacterial Infections / immunology
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Gram-Negative Bacterial Infections / microbiology
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Gram-Negative Bacterial Infections / prevention & control
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Gram-Negative Bacterial Infections / veterinary*
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Polymerase Chain Reaction / veterinary
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Random Allocation
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Vaccination / methods
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Vaccination / veterinary
Substances
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Antibodies, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Vaccines
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DNA, Bacterial
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Drugs, Chinese Herbal
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