Complete sequence of the suicide vector pJP5603

Thomas Riedel; Meike Rohlfs; Ina Buchholz; Irene Wagner-Döbler; Michael Reck

doi:10.1016/j.plasmid.2012.07.005

Complete sequence of the suicide vector pJP5603

Plasmid. 2013 Jan;69(1):104-7. doi: 10.1016/j.plasmid.2012.07.005. Epub 2012 Aug 9.

Authors

Thomas Riedel¹, Meike Rohlfs, Ina Buchholz, Irene Wagner-Döbler, Michael Reck

Affiliation

¹ Helmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany. tri07@helmholtz-hzi.de

PMID: 22902299
DOI: 10.1016/j.plasmid.2012.07.005

Abstract

We have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / metabolism
Base Sequence*
Chromosomes / genetics
Cloning, Molecular / methods
DNA Replication
Escherichia coli / genetics*
Escherichia coli / metabolism
Genetic Vectors / genetics*
Homologous Recombination
Kanamycin / metabolism
Lac Operon
Mutagenesis, Site-Directed
Plasmids / genetics

Substances

Bacterial Proteins
Kanamycin