Quantification of the antioxidant activity in salt-stressed tissues

Methods Mol Biol. 2012:913:237-50. doi: 10.1007/978-1-61779-986-0_16.

Abstract

Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu's procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / metabolism*
  • Enzyme Assays / methods
  • Phenols / chemistry
  • Plant Extracts / chemistry
  • Plant Extracts / metabolism
  • Plants / chemistry
  • Plants / enzymology
  • Plants / metabolism*
  • Salinity*
  • Salt Tolerance / physiology*
  • Stress, Physiological*

Substances

  • Antioxidants
  • Phenols
  • Plant Extracts