Remote chlorophyll fluorescence measurements with the laser-induced fluorescence transient approach

Methods Mol Biol. 2012:918:51-9. doi: 10.1007/978-1-61779-995-2_5.

Abstract

The interaction of plants with their environment is very dynamic. Studying the underlying processes is important for understanding and modeling plant response to changing environmental conditions. Photosynthesis varies largely between different plants and at different locations within a canopy of a single plant. Thus, continuous and spatially distributed monitoring is necessary to assess the dynamic response of photosynthesis to the environment. Limited scale of observation with portable instrumentation makes it difficult to examine large numbers of plants under different environmental conditions. We report here on the application of a recently developed technique, laser-induced fluorescence transient (LIFT), for continuous remote measurement of photosynthetic efficiency of selected leaves at a distance of up to 50 m. The ability to make continuous, automatic, and remote measurements of photosynthetic efficiency of leaves with the LIFT provides a new approach for studying the interaction of plants with the environment and may become an important tool in phenotyping photosynthetic properties in field applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Chlorophyll / metabolism*
  • Fluorescence*
  • Lasers*
  • Models, Biological
  • Spectrometry, Fluorescence / instrumentation*
  • Time Factors

Substances

  • Chlorophyll