Engineering of therapeutic polypeptides through chemical synthesis: early lessons from human parathyroid hormone and analogues

J Am Chem Soc. 2012 Sep 12;134(36):15122-9. doi: 10.1021/ja306637u. Epub 2012 Aug 29.

Abstract

Application of chemical synthesis to gain access to high purity hPTH as well as more stable analogues was accomplished through a menu of extended NCL followed by metal free dethiylation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Calcium / blood
  • Calcium / metabolism
  • HEK293 Cells
  • Humans
  • Injections, Subcutaneous
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Structure
  • Parathyroid Hormone / administration & dosage
  • Parathyroid Hormone / chemical synthesis*
  • Parathyroid Hormone / pharmacology
  • Peptides / administration & dosage
  • Peptides / chemical synthesis*
  • Peptides / pharmacology
  • Protein Engineering*

Substances

  • Parathyroid Hormone
  • Peptides
  • Calcium