Very-long-distance correlations in proteins revealed by solid-state NMR spectroscopy

Chemphyschem. 2012 Nov 12;13(16):3585-8. doi: 10.1002/cphc.201200548. Epub 2012 Aug 13.

Abstract

I still see you: a new pulse sequence, SHA+, little sensitive to dipolar truncation, allows direct or relayed polarization transfer between (13)C atoms, distant by 3.5-9.6 Å, in amyloid fibrils. SHA+ can also be used in a broadband way with the weak rf-condition of ν(1)/ν(R)≈0.2-0.3 which permits the investigation of temperature-sensitive biological systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / chemistry*
  • Humans
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Peptide Fragments / chemistry*
  • Protein Conformation
  • Temperature

Substances

  • Amyloid beta-Peptides
  • Peptide Fragments
  • amyloid beta-protein (1-42)