Abstract
A new gut bacterial adhesion model has been developed. For this, a continuous-flow bioreactor packed with bacteria-coated beads was designed to simulate the gut lining and other features. In vitro model efficacy shows successful bacterial cell gut adhesions: bacterial adhesion was higher with mucin-alginate compared to controls. In feasibility study, adhesion of Lactobacillus fermentum NCIMB 5221 and Lactobacillus reuteri NCIMB 701359 was investigated for their metabolic activities for bile salt. Bile salt hydrolase (BSH)-active Lactobacillus reuteri exerted higher activity than non-BSH-active L. fermentum. This model has potential use in gut health, probiotic, bacterial cell gut adhesion and other delivery applications.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alginates / chemistry
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Alginates / pharmacology
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Amidohydrolases / metabolism*
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Bacterial Adhesion
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Bacterial Proteins / metabolism*
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Bile Acids and Salts / metabolism*
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Bioreactors
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Gastrointestinal Tract / microbiology
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Glucuronic Acid / chemistry
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Glucuronic Acid / pharmacology
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Hexuronic Acids / chemistry
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Hexuronic Acids / pharmacology
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Humans
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Limosilactobacillus fermentum / drug effects
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Limosilactobacillus fermentum / growth & development*
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Limosilactobacillus reuteri / drug effects
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Limosilactobacillus reuteri / enzymology
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Limosilactobacillus reuteri / growth & development*
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Models, Anatomic*
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Mucins / chemistry
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Mucins / pharmacology
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Probiotics / metabolism*
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Species Specificity
Substances
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Alginates
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Bacterial Proteins
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Bile Acids and Salts
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Hexuronic Acids
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Mucins
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Glucuronic Acid
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Amidohydrolases
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choloylglycine hydrolase