Facile graft of poly(2-methacryloyloxyethyl phosphorylcholine) onto Fe(3) O(4) nanoparticles by ATRP: synthesis, properties, and biocompatibility

J Biomed Mater Res A. 2013 Feb;101(2):607-12. doi: 10.1002/jbm.a.34343. Epub 2012 Aug 10.

Abstract

Magnetite (Fe(3) O(4) ) nanoparticles with the surface hydroxyl groups were achieved by a polyol process. Using 2-(4-chlorosulfonylphenyl) ethyltrichlorosilane (CTCS) as initiator, 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) as monomer, poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC)-grafted Fe(3) O(4) nanoparticles (MNP) were successfully prepared via the atom transfer radical polymerization (ATRP) method. The successful grafting of PMPC on the Fe(3) O(4) nanoparticles surface was ascertained from the FTIR analysis. The modified nanoparticles (MNP-CTCS-PMPC) showed a good biocompatibility in the cytotoxicity test in vitro. Performance testing of MNP-CTCS-PMPC was performed through magnetic resonance analysis (MR), and its r(2) /r(1) value was 24.1. These results indicated that the modified Fe(3) O(4) nanoparticles would be a potential MRI contrast reagent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ferric Compounds / chemical synthesis*
  • Ferric Compounds / chemistry*
  • HeLa Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • Materials Testing*
  • Methacrylates / chemical synthesis*
  • Methacrylates / chemistry*
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Phosphorylcholine / analogs & derivatives*
  • Phosphorylcholine / chemical synthesis
  • Phosphorylcholine / chemistry
  • Polymerization*
  • Polymethacrylic Acids
  • Silanes / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Thermogravimetry
  • X-Ray Diffraction

Substances

  • Ferric Compounds
  • Methacrylates
  • Polymethacrylic Acids
  • Silanes
  • poly(2-methacryloyloxyethyl-phosphorylcholine)
  • Phosphorylcholine
  • ferric oxide