A simple, sensitive method for the analysis of flutriafol in wheat and soil was developed using high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The dissipation dynamics and ultimate residue of flutriafol in wheat and soil were detected using the established method. The half-lives of flutriafol in wheat plant were 4.09 days (Hebei) and 1.54 days (Hubei), while in soil were 6.25 days (Hebei) and 3.16 days (Hubei), respectively. The final residues of flutriafol were below 0.5 mg/kg (MRL of EU) in wheat grain 28 days after the treatment.