Optimization of the URA-blaster disruption system in Candida guilliermondii: efficient gene targeting using the URA3 marker

Emilien Foureau; Vincent Courdavault; Andrew J Simkin; Olivier Pichon; Joël Crèche; Nathalie Giglioli-Guivarc'h; Marc Clastre; Nicolas Papon

doi:10.1016/j.mimet.2012.07.020

Optimization of the URA-blaster disruption system in Candida guilliermondii: efficient gene targeting using the URA3 marker

J Microbiol Methods. 2012 Oct;91(1):117-20. doi: 10.1016/j.mimet.2012.07.020. Epub 2012 Aug 3.

Authors

Emilien Foureau¹, Vincent Courdavault, Andrew J Simkin, Olivier Pichon, Joël Crèche, Nathalie Giglioli-Guivarc'h, Marc Clastre, Nicolas Papon

Affiliation

¹ EA2106, Biomolécules et Biotechnologies Végétales, UFR Pharmacie, Université François Rabelais de Tours, France.

PMID: 22884441
DOI: 10.1016/j.mimet.2012.07.020

Abstract

We established a simple transformation system for C. guilliermondii by developing both an ura3 ATCC 6260-derived recipient strain as well as an URA3 blaster cassette. We demonstrated that this strategy allows efficient multiple gene disruption by homologous recombination with a convenient gene targeting frequency.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Candida / genetics*
Fungal Proteins / genetics
Fungal Proteins / metabolism
Gene Targeting / methods*
Recombination, Genetic
Selection, Genetic

Substances

Fungal Proteins